Goat anti-AMHR2 (aa88-99) Antibody

• Catalog Number: orb233603
• Category: Antibodies
• Description: Goat polyclonal antibody to AMHR2
• Target: AMHR2 (aa88-99)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESLHCDPSPRAH
• MW: 62.7; 52.2; 51.8
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml
• Application notes: This antibody is expected to recognize all reported isoformsWB: Approx 60+65kDa bands observed in lysates of cell line MDA-MB-231
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Anti-Muellerian hormone type II receptor [Pre
• Note: For research use only
• Entrez: 269

1 μg/ml staining of MCF7 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.