Goat anti-AMHR2 (aa88-99) Antibody

SKU: orb233603

Description

Goat polyclonal antibody to AMHR2

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml
• Reactivity: Human
• Application Notes:
  This antibody is expected to recognize all reported isoformsWB: Approx 60+65kDa bands observed in lysates of cell line MDA-MB-231

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AMHR2 (aa88-99)
• Molecular Weight: 62.7; 52.2; 51.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

Anti-Anti-Muellerian hormone type II receptor [Precursor] antibody, anti-MISRII antibody, anti-MRII antibody, anti-AMHR2 antibody, anti-AMHR antibody

1 μg/ml staining of MCF7 cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

AMHR2 (aa88-99)

NCBI Gene ID

269