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Catalog Number | orb20099 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to ALOX15 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:32000, WB: 0.2-0.5 μg/ml, IHC-P: 2 μg/ml |
Conjugation | Unconjugated |
MW | 74.8 |
Target | ALOX15 |
Entrez | 246 |
Protein Sequence | HYKTDVAVKDDPE |
RRID | AB_10753739 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | Anti-15 LOX antibody, anti-15-LOX antibody, anti-1 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20099 (0.2ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.