GNB1 Antibody

• Catalog Number: orb381069
• Category: Antibodies
• Description: GNB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Bovine, Canine, Equine, Hamster, Monkey, Rabbit, Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GNB1 (2-42aa SELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGR), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Flow Cytometry, 1-3μg/1x106 cells, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 37377 MW
• UniProt ID: P62873
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Guanine nucleotide-binding protein G (I)/G (S)/G (
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-GNB1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of GNB1 using anti-GNB1 antibody.Lane 1:human HeLa cell;2:human MCF-7 cell;3:human SiHa cell;4:rat brain tissue;5:rat liver tissue;6:mouse brain tissue;7:mouse liver tissue.

IF analysis of GNB1 using anti-GNB1 antibody.GNB1 was detected in immunocytochemical section of A549 cell.

IHC analysis of GNB1 using anti-GNB1 antibody.GNB1 was detected in paraffin-embedded section of mouse kidney tissue.

IHC analysis of GNB1 using anti-GNB1 antibody.GNB1 was detected in paraffin-embedded section of rat kidney tissue.

IHC analysis of GNB1 using anti-GNB1 antibody.GNB1 was detected in paraffin-embedded section of human liver cancer tissue.

IHC analysis of GNB1 using anti-GNB1 antibody.GNB1 was detected in frozen section of human placenta tissue.