GM2A Antibody

• Catalog Number: orb1249254
• Category: Antibodies
• Description: GM2A Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Canine, Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: KRLGCIKIAAS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Approx. 24kDa band was observed in Rat Kidney lysates (calculated MW of 21.5kDa according to NP_758838.2 ). Recommended concentration: 0.3-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GM2A
• UniProt ID: P17900
• NCBI: NP_000396.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: GM2A, GM2 ganglioside activator, SAP-3, cerebrosid
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Approx. 24kDa band was observed in Rat Kidney lysates (calculated MW of 21.5kDa according to NP_758838.2 ). Recommended concentration: 0.3-1ug/ml.Purchase the +ve control! The transfected lysate used to QC this product is available for sale. Please email [email protected] to request datasheet, pricing and delivery information.
• Expiration Date: 12 months from date of receipt.

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human GM2A with DYKDDDDK tag probed with orb1249254 (1.0 ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1/30000) in lane C. Mock-transfected HEK293 probed with orb1249254 (1 mg/ml) in Lane B.

orb1249254 (0.3 ug/ml) staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.