Glypican-1 antibody

• Catalog Number: orb344647
• Category: Antibodies
• Description: Glypican-1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
• Concentration: 2.1 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 10µg/mL, WB: 1:500- 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Glypican-1 was protein A purified from monospecific antiserum by immunoaffinity chromatography using protein A coupled to agarose beads. This antibody is specific for human glypican-1 protein. A BLAST analysis was used to suggest partial cross-reactivity with glypican from rat, mouse, Macaque, dog, cattle, and opossum sources based on 100 - 88% homology with the immunizing sequence. Cross-reactivity with glypican from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P35052
• NCBI: 30410985
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Glypican-1 Antibody, Glypican 1, Glypi
• Note: For research use only
• Application notes: Anti-Glypican-1 protein A purified antibody has been tested for use in ELISA, and immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kDa in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kDa) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane using Glypican-1 antibody