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Catalog Number | orb215958 |
---|---|
Category | Antibodies |
Description | Glutamate Receptor 1/GRIA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Predicted Reactivity | Bovine, Canine, Equine, Monkey, Rabbit |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human GRIA1 recombinant protein (Position: A19-R360). Human GRIA1 shares 98% amino acid (aa) sequence identity with both mouse and rat GRIA1. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 101506 MW |
UniProt ID | P42261 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Glutamate receptor 1;GluR-1;AMPA-selective glutama Read more... |
Note | For research use only |
Application notes | WB: The detection limit for GRIA1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of GRIA1 using anti-GRIA1 antibody.Lane 1:Recombinant Human GRIA1 Protein 0.5ng.
WB analysis of GRIA1 using anti-GRIA1 antibody.Lane 1:Rat Brain Tissue;2:Mouse Brain Tissue.
IHC analysis of GRIA1 using anti-GRIA1 antibody.GRIA1 was detected in paraffin-embedded section of Mouse Brain Tissue.
IHC analysis of GRIA1 using anti-GRIA1 antibody.GRIA1 was detected in paraffin-embedded section of Rat Brain Tissue.
IHC analysis of GRIA1 using anti-GRIA1 antibody.GRIA1 was detected in paraffin-embedded section of Human Meningeoma Tissue.
IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rat, Sheep |
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