Glucocorticoid Receptor/NR3C1 Antibody

• Catalog Number: orb215986
• Category: Antibodies
• Description: Glucocorticoid Receptor/NR3C1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human NR3C1 recombinant protein (Position: M1-D373). Human NR3C1 shares 85% and 82% amino acid (aa) sequences identity with mouse and rat NR3C1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85659 MW
• UniProt ID: P04150
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glucocorticoid receptor;GR;Nuclear receptor subfam
• Note: For research use only
• Application notes: WB: The detection limit for NR3C1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-NR3C1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of NR3C1 using anti-NR3C1 antibody.Lane 1:human HeLa cell; 2:human A549 cell; 3:human HEK293 cell.

IF analysis of NR3C1 using anti- NR3C1 antibody. NR3C1 was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in paraffin-embedded section of human gastric cancer tissue.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in paraffin-embedded section of human gastric cancer tissue.

IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in paraffin-embedded section of human rectal cancer tissue.