Glucoamylase antibody (Biotin)

• Catalog Number: orb344316
• Category: Antibodies
• Description: Glucoamylase antibody (Biotin)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Other
• Isotype: IgG
• Immunogen: Glucoamylase [Rhizopus]
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,500
• Form/Appearance: Lyophilized
• Purity: Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown.
• Conjugation: Biotin
• UniProt ID: P07683
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Glucoamylase Antibody biotin Conjugation
• Note: For research use only
• Application notes: Anti-Glucoamylase biotin has been tested by ELISA and western blot. This product is assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:75,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.