GLI2 Antibody (Internal)

• Catalog Number: orb1539019
• Category: Antibodies
• Description: GLI2 Antibody (Internal)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.
• Concentration: 1 mg/ml
• Dilution range: ELISA (1:15000 - 1:60000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: GLI2
• Storage: Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
• Buffer/Preservatives: 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
• Alternative names: GLI2, GLI-Kruppel family member GLI2, HPE9, Gli-2,
• Note: For research use only
• Application notes: Further information: Immunohistochemistry: LS-B75 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B75 was determined to be 5 ug/ml.
• Expiration Date: 12 months from date of receipt.