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Catalog Number | orb2292898 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant GLI1. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1G11 |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Isotype | IgG1 Kappa |
Immunogen | GLI1 (AAH13000, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Conjugation | Unconjugated |
Protein Sequence | GPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLEN |
NCBI | AAH13000 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Detection limit for recombinant GST tagged GLI1 is 0.03 ng/ml as a capture antibody.
GLI1 monoclonal antibody (M02), clone 1G11. Western Blot analysis of GLI1 expression in HeLa.
Immunofluorescence of monoclonal antibody to GLI1 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa).