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Catalog Number | orb334005 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to GLI1 |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 1B9F8 |
Tested applications | FC, ICC, IHC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Purified recombinant fragment of human GLI1 (AA: 284-449) expressed in E. Coli. |
Antibody Type | Primary Antibody |
Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
Dilution range | IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
Conjugation | Unconjugated |
MW | 118kDa |
Target | GLI1 |
Entrez | 2735 |
UniProt ID | P08151 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
Alternative names | GLI Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hela cells using GLI1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded ovarian cancer tissues using GLI1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using GLI1 antibody
Western blot analysis of human recombinant protein using GLI1 antibody
Western blot analysis of HEK293 using GLI1 antibody
ELISA Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
Flow cytometric analysis of HepG2 cells using GLI1 antibody
FC, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |