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Catalog Number | orb1252545 |
---|---|
Category | Antibodies |
Description | GLG1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | GLG1/970 |
Tested applications | FC, ICC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | IgG1, kappa |
Immunogen | The Golgi fraction from human liver cells was used as the immunogen for the Golgi Marker antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 ug/ml for 30 minWestern blot: 0.5-1.0 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Golgi Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | GLG1 |
UniProt ID | Q92896 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Golgi apparatus protein 1, CFR-1, Cysteine-rich fi Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1 ug/ml for 30 minWestern blot: 0.5-1.0 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Golgi Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human pancreas stained with Golgi Marker antibody (GLG1/970).
IHC-P, WB | |
Gallus, Hamster, Rat | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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