GJA1 antibody

• Catalog Number: orb393297
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GJA1.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Primate
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Connexin 43. The exact sequence is proprietary.
• Dilution range: WB: 1:500:1000, IF/ICC: 1:100:500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: GJA1
• Entrez: 2697
• UniProt ID: P17302
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: GJAL; Gap junction alpha-1 protein; Connexin-43; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Connexin 43 expression in HeLa (A), MCF7 (B) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

Immunofluorescent analysis of Connexin 43 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).