GJA1 Antibody

• Catalog Number: orb1267871
• Category: Antibodies
• Description: GJA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Bovine, Xenopus
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit Ig
• Immunogen: This Connexin 43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-138 amino acids from the N-terminal region of human Connexin 43.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: GJA1
• UniProt ID: P17302
• NCBI: P17302
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Gap junction alpha-1 protein, Connexin-43, Cx43, G
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from Hela, U-87 MG, C6 cell line and mouse heart tissue lysate (from left to right), using GJA1 Antibody (N121) at 1:1000 at each lane.

(LEFT) Western blot analysis of anti-GJA1 Antibody (N-term) in CEM cell line lysates (35 ug/lane). GJA1 (arrow) was detected using the purified Pab. (RIGHT) Western blot analysis of hGJA1-N121.Connexin in mouse brain tissue lysates (35 ug/lane).GJA1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.