GITR Antibody [YGITR765], Rabbit IgG

• Catalog Number: orb1671931
• Category: Antibodies
• Description: GITR Antibody [YGITR765], Rabbit IgG
• Clonality: Recombinant
• Clone Number: YGITR765
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: nB2/6TG cells transfected with mouse GITR injected into DA rats.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD357, Tumor necrosis factor receptor superfamily
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed mouse splenocytes with anti-GITR antibody YGITR765. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of YGITR765 (orb1671931) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671931- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor® 488 secondary antibody.

Flow-cytometry using anti-GITR antibody YGITR765. Mouse activated T cells were stained with an isotype control (3.0 - Orange) or the rmeric version of YGITR765 (orb1671931) at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing- bound antibody was detected using a AF488 conjugated donkey anti-ribody and cells analysed on a FACSCanto flow-cytometer.