GIPC1 antibody

• Catalog Number: orb18596
• Category: Antibodies
• Description: Goat polyclonal antibody to GIPC1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Dilution range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml
• Conjugation: Unconjugated
• MW: 36.0; 26.1
• Target: GIPC1 / NIP
• Entrez: 10755
• Protein Sequence: GAIGDAKVGRY
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GIPC1 antibody, anti NIP antibody, anti GIPC
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18596 staining (0.03ug/ml) of human HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing GIPC1 isoform 1 (RC216466) and probed with orb18596 (mock transfection in first lane), tested by Origene.