GFP Mouse Monoclonal Antibody

SKU: orb499984

Description

GFP Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:50000-500000, ELISA=1:1000-5000, IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000, ICC/IF=1:100-500, Flow-Cyt=1ug/Test
• Reactivity: All

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 4G6
• Immunogen: Recombinant Green Fluorescence Protein
• Target: GFP
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GFP_AEQVI; GFP;

Sample: GFP-Tagged Fusion Protein Overexpression E.coli Lysate (orb1516874) at 2 ug, Primary: Anti-GST tag (orb499984) at 1/5000 ~ 1/10000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 28 kD.

Sample: GFP-tagged fusion protein Overexpression E.coli Lysate (orb1516874) at 4 ug, Primary: Anti-GFP-Tag (orb499984) at 1/1000000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 28 kD, Observed band size: 30 kD.

Transformed (GFP or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with orb499984 (Anti-GFP) at dilution of 1:1000000 incubated at 4°C overnight.

Quick Database Links

Gene Symbol

GFP