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Catalog Number | orb666764 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to GFAT2 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Recombinant fusion protein of human GFAT2. The exact sequence is proprietary. |
Dilution range | WB: 1-500-2000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | GFPT2 |
Entrez | 360518, 14584, 9945 |
UniProt ID | Q9Z2Z9, O94808, Q4KMC4 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | Anti-Glutamine--fructose-6-phosphate aminotransfer Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of GFAT2 expression in A375 (A), NIH3T3 (B), mouse testis (C), rat heart (D) whole cell lysates. (Predicted band size: 76 kD; Observed band size: 77 kD)
Immunofluorescent analysis of GFAT2 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ICC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
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