GFAP R416WT antibody

• Catalog Number: orb150545
• Category: Antibodies
• Description: Mouse monoclonal to GFAP R416WT (HRP). The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proiliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S206B-9
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1
• Immunogen: Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP; 100% identical in rat and mouse. > 50% identity with other proteins (Vimentin, Desmin and Peripherin).
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: HRP
• MW: 50kDa
• Target: GFAP
• Entrez: 2670
• UniProt ID: P14136
• NCBI: NP_001124491.1
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated
• Alternative names: Glial fibrillary acidic protein antibody, Intermed
• Note: For research use only
• Application notes: 1 µg/ml of SMC-442 was sufficient for detection of GFAP R416WT in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.