GFAP Antibody (monoclonal, 3F2)

• Catalog Number: orb623781
• Category: Antibodies
• Description: GFAP Antibody (monoclonal, 3F2)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3F2
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1
• Immunogen: E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse, rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat Immunofluorescence, 5 μg/ml, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 50 kDa
• UniProt ID: P14136
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

IF analysis of Histone H3 and GFAP using anti-Histone H3 antibody and anti-GFAP antibody.

IHC analysis of GFAP using anti-GFAP antibody.

IHC analysis of GFAP using anti-GFAP antibody.

WB analysis using anti-GFAP antibody.Lane 1:rat brain tissue, Lane 2:mouse brain tissue.