GFAP Antibody / Glial Fibrillary Acidic Protein [Out of stock]

• Catalog Number: orb750113
• Category: Antibodies
• Description: Glial fibrillary acidic protein is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [RefSeq]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFA12-3
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Native Glial fibrillary acidic protein was used as the immunogen for this GFAP antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This GFAP antibody is available for research use only.
• Entrez: 2670
• Storage: Store the GFAP antibody at 2-8°C.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the GFAP antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human brain and GFAP antibody (clone GFA12-3). Predicted molecular weight ~50 kDa.

IHC testing of FFPE human brain and GFAP antibody (clone GFA12-3). FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.