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| Catalog Number | orb1806557 |
|---|---|
| Category | Assays and Kits |
| Description | General Vanillylmandelic Acid (VMA) ELISA Kit |
| Target | VMA |
| Reactivity | All |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Vanillylmandelic Acid(VMA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vanillylmandelic Acid(VMA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vanillylmandelic Acid(VMA) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Assay Type | Competitive |
| Assay Time | 1.5H |
| Standard | 10000 ng/mL |
| Range | 156.25-10000 ng/mL |
| Sensitivity | 43.7 ng/mL |
| Sample Types | urine and biological agents |
| Alternative names | Vanillyl Mandelic Acid |
| Note | For research use only |
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