General SIP(Sphingosine 1 Phosphate) ELISA Kit

• Catalog Number: orb1146848
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine 1 Phosphate(SIP). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sphingosine 1 Phosphate(SIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine 1 Phosphate(SIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine 1 Phosphate(SIP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: All
• Range: 3.13-200 ng/mL
• Sensitivity: 0.91 ng/mL
• Protein Length: 3.5h
• Alternative names: EDG3, LPB3, S1P3, S1PR3, endothelial differentiati
• Note: For research use only
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Expiration Date: Please enquire.