Gelsolin/GSN Antibody

• Catalog Number: orb137912
• Category: Antibodies
• Description: Gelsolin/GSN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Monkey, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin (763-775aa WDDDYWSVDPLDR), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Monkey, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By HeatImmunocytochemistry , 0.5-1μg/ml, Mouse Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 85698 MW
• UniProt ID: P06396
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Gelsolin;AGEL;Actin-depolymerizing factor;ADF;Brev
• Note: For research use only
• Application notes: WB: The detection limit for GSN is approximately 1.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: HELA Cell Lysate, Lane 2: A431 Cell Lysate using GSN antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Cardiac Muscle Tissue using GSN antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Lung Cancer Tissue using GSN antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Intestinal Cancer Tissue using GSN antibody

Confocal immunofluoroscence analysis of NIH3T3 cells using GSN antibody