GDNF Receptor alpha 1/GFRA1 Antibody

• Catalog Number: orb215956
• Category: Antibodies
• Description: GDNF Receptor alpha 1/GFRA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GFRA1 recombinant protein (Position: D25-Q227). Human GFRA1 shares 97% amino acid (aa) sequence identity with both mouse and rat GFRA1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 51456 MW
• UniProt ID: P56159
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: GDNF family receptor alpha-1;GDNF receptor alpha-1
• Note: For research use only
• Application notes: WB: The detection limit for GFRA1 is approximately 0.2ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of GFRA1 using anti-GFRA1 antibody.Lane 1:recombinant human GFRA1 protein 0.5 ng.

WB analysis of GFRA1 using anti-GFRA1 antibody.Lane 1:human placenta tissue.

IHC analysis of GFRA1 using anti-GFRA1 antibody. GFRA1 was detected in a paraffin-embedded section of human lung cancer tissue.