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Catalog Number | orb1539153 |
---|---|
Category | Antibodies |
Description | GDF15 Antibody (C-Terminus, clone 6D12.H10.E4) |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | 6D12.H10.E4 |
Tested applications | ELISA, IHC, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG2a,l |
Immunogen | This Protein-A purified antibody was prepared by repeated immunizations with an MBP-tagged recombinant protein produced in E.coli corresponding to C-term mouse NAG-1 protein. Cross reactivity to MBP is negative. |
Concentration | 1.09 mg/ml |
Dilution range | ELISA (1:2000), IHC, IHC-P (10 µg/ml), WB (1:1000) |
Conjugation | Unconjugated |
Target | GDF15 |
Storage | Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. |
Buffer/Preservatives | 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide. 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide |
Alternative names | GDF15, MIC1, MIC-1, NAG-1, NRG-1, NSAID-regulated Read more... |
Note | For research use only |
Application notes | Further information: This Protein G purified antibody is suitable for ELISA and western blotting of human NAG-1 protein. For detection of NAG-1 in human serum, a sandwich ELISA is suggested using this antibody in combination with anti-NAG-1/GDF15 (N-terminal), H variant or D variant specific antibodies. Specific conditions for reactivity should be optimized by the end user. Expect bands in Western blots of native protein of approximately 13 and 26 kD in size corresponding to NAG-1 monomer and dimer, respectively, using the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
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