GCPII antibody

• Catalog Number: orb43794
• Category: Antibodies
• Description: Mouse Monoclonal to GCPII.
• Clonality: Monoclonal
• Clone Number: GCP-04
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Isotype: Mouse IgG1
• Immunogen: Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells
• Concentration: 1 mg/ml
• Dilution range: Western blotting: Recommended dilution: 1 μg/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: PSMA
• Entrez: 2346
• UniProt ID: Q04609
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-GCPII antibody
• Note: For research use only
• Application notes: Western blotting: Recommended dilution: 1 μg/ml; positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer. Both reducing and non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of GCPII in human medulla oblongata by GCP-04 monoclonal antibody. Mag. 40x; positive astrocytes in white matter.

Immunohistochemistry staining of GCPII in human prostate by GCP-04 monoclonal antibody. Mag. 400x; positive epithelium of the prostate glands.

Immunohistochemistry staining of GCPII in porcine kidney by GCP-04 monoclonal antibody. Highly positive proximal glomeruli.

Western blotting analysis of human PSMA using mouse monoclonal antibody GCP-04 on lysates of LNCaP cell line and Jurkat cell line (PSMA non-expressing cell line; negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-PSMA monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody.