GCNT3 antibody

• Catalog Number: orb19758
• Category: Antibodies
• Description: Goat polyclonal antibody to GCNT3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:2000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 50.9
• Target: C2GnT-M (aa 273 to 284)
• Entrez: 9245
• Protein Sequence: RDTLHLTNKKKD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GCNT3 antibody, anti glucosaminyl (N-acetyl)
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx. 50kDa band observed in Human Lymph Node lysates (calculated MW of 50.9kDa according to NP_004742.1). In transfected HEK293 transiently expressing GCNT3 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19758 (0.3ug/ml) staining of human lymph node lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing GCNT3 (RC202007) and probed with orb19758 (mock transfection in first lane), tested by Origene.