GCN2 (phospho-Thr899) antibody

• Catalog Number: orb6078
• Category: Tools
• Description: GCN2 (phospho-Thr899) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Bovine, Canine, Equine, Rat
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human GCN2 around the phosphorylation site of Thr899 (HL(p-T)GM)
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1ug/Test, WB=1:500-2000, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 189 kDa
• Target: Phospho-GCN2 (Thr899)
• UniProt ID: Q9P2K8
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: GCN2 (phospho T899); p-GCN2 (phospho T899); EIF2AK
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of rat brain lysates(Lane 1), rat kidney lysates (Lane 2) using GCN2 (phospho-Thr899) antibody at 1:200 dilution

Paraffin-embedded mouse liver tissue fixed with 4% paraformaldehyde.Antigen retrieval by boiling with citrate buffer.Blocking buffer is goat serum (37'C for 20min) Phospho-GCN2 (Thr899) antibody at 1:200 with overnight incubation at 4 degrees

Paraffin-embedded mouse small intestine tissue fixed with 4% paraformaldehyde.Antigen retrieval by boiling with citrate buffer.Blocking buffer is goat serum (37'C for 20min) Phospho-GCN2(Thr899)antibody at:1:200 with overnight incubation at 4 degrees

Immunohistochemical analysis of paraffin embedded rat ovarian tissue using GCN2 (phospho-Thr899) antibody