GCN1L1 Antibody

• Catalog Number: orb1527689
• Category: Antibodies
• Description: GCN1L1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: between 2621 and 2671
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GCN1L1
• UniProt ID: Q92616
• NCBI: NP_006827.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: GCN1-like protein 1; PRIC295; translational activa
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse and rat GCN1L1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, and C6 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GCN1L1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, RPMI-8226, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GCN1L1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GCN1L1 antibody orb1527689 used for IP at 6 µg per reaction. GCN1L1 was also immunoprecipitated by a previous lot of this antibody (orb1527689).