GCET2 Monoclonal Antibody

• Catalog Number: orb548493
• Category: Antibodies
• Description: GCET2 Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: AFHF-7
• Tested applications: FC, ICC, IF, IHC
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: IHC 1:100-1:500ICC/IF 1:100-1:500FC 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• UniProt ID: Q8N6F7
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC analysis of GCSAM using anti-GCSAM antibody (orb548493). GCSAM was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GCSAM Antibody (orb548493) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.