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    GCC1 Antibody

    Catalog Number: orb865396

    DispatchUsually dispatched within 5-10 working days
    $ 520.00
    Catalog Numberorb865396
    CategoryAntibodies
    DescriptionGCC1 Antibody
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsELISA, ICC, IF, WB
    ReactivityHuman, Mouse, Rat
    IsotypeRabbit IgG
    ImmunogenE.coli-derived human GCC1 recombinant protein (Position: E22-L763).
    ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Dilution rangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Direct ELISA, 0.1-0.5 μg/ml, Human
    Form/AppearanceLyophilized
    ConjugationUnconjugated
    MW100 kDa
    UniProt IDQ96CN9
    StorageAt -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
    NoteFor research use only
    Application notesTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
    Expiration Date12 months from date of receipt.
    GCC1 Antibody

    Western blot analysis of GCC1 using anti-GCC1 antibody (orb865396). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse Neuro-2a whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: rat ovary tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCC1 antigen affinity purified polyclonal antibody (Catalog # orb865396) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for GCC1 at approximately 100 kDa. The expected band size for GCC1 is at 100 kDa.

    GCC1 Antibody

    IF analysis of GCC1 using anti-GCC1 antibody (orb865396). GCC1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-GCC1 Antibody (orb865396) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

    • GCC1 antibody [orb35765]

      ELISA,  IHC,  WB

      Human

      Rabbit

      Polyclonal

      Unconjugated

      50 μg, 100 μg
    • GCC1 Antibody [orb1269251]

      IHC-P,  WB

      Human

      Rabbit

      Polyclonal

      Unconjugated

      400 μl
    • GCC1 antibody [orb678462]

      ELISA,  WB

      Human, Mouse, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
    • GCC1 antibody (HRP) [orb35766]

      ELISA

      Human

      Rabbit

      Polyclonal

      HRP

      50 μg, 100 μg
    • GCC1 antibody (FITC) [orb35767]

      Human

      Rabbit

      Polyclonal

      FITC

      100 μg, 50 μg
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