GC1q R/C1QBP Antibody

• Catalog Number: orb371655
• Category: Antibodies
• Description: GC1q R/C1QBP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human GC1q R recombinant protein (Position: E190-Q282). Human GC1q R shares 92.5% and 93.5% amino acid (aa) sequence identity with mouse and rat GC1q R, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 31362 MW
• UniProt ID: Q07021
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Complement component 1 Q subcomponent-binding prot
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-GC1q R antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of C1QBP using anti-C1QBP antibody.Lane 1:human HeLa cell; 2:human SW620 cell; 3:human 293T cell; 4:human A431 cell; 5:rat brain tissue; 6:rat spleen tissue; 7:mouse brain tissue; 8:mouse spleen tissue.

IF analysis of C1QBP using anti-C1QBP antibody.C1QBP was detected in immunocytochemical section of U20S cell.

IHC analysis of C1QBP using anti-C1QBP antibody.C1QBP was detected in paraffin-embedded section of human tonsil tissues.

IHC analysis of C1QBP using anti-C1QBP antibody.C1QBP was detected in paraffin-embedded section of mouse spleen tissues.

IHC analysis of C1QBP using anti-C1QBP antibody.C1QBP was detected in paraffin-embedded section of rat spleen tissues.