GC antibody

• Catalog Number: orb1427645
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GC.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21567
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Porcine
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:2000, WB: 1:1000, IHC-P-Leica: 1:500, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 59716
• Target: GBA
• UniProt ID: P04062
• NCBI: NP_001005742.1, NP_000148.2, NP_001005741.1
• Alternative names: Glucosylceramidase, Acid beta-glucosidase, Algluce
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot:All lanes:Anti-GC Antibody (Center) at 1:2000 dilution.Lane 1:293T/17;Lane 2:Hela;Lane 3:LNCaP;Lane 4:MCF-7;Lane 5:U-2OS.

Western blot analysis of GC Antibody (Center) in mouse lung tissue lysates.GC (arrow) was detected using the purified Pab.

Immunohistochemical analysis of human skeletal muscle tissue using AP8937C performed on the Leica® BOND RXm at 1:500 dilution.A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow cytometric analysis of Hela cells stained with orb1427645(green line) at 1:25 dilution.Secondary antibody was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed.Isotype control antibody (blue line) was rabbit IgG1.