GBF1 Antibody

• Catalog Number: orb1260863
• Category: Antibodies
• Description: GBF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 260kDa
• Target: GBF1
• UniProt ID: Q92538
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: ARF1GEF, Golgi-specific brefeldin A-resistance gua
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of HeLa cells, using GBF1 Antibody (orb1260863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.