GATA4 Antibody

SKU: orb344533

Description

GATA4 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:2000-1:10,000, WB: 1:500-1:1000
• Reactivity: Human
• Application Notes:
  Anti-GATA4 antibody has been tested by ELISA, SDS-Page, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 10D8.B10.D4
• Immunogen: GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
• Purity: Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography. A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody

Western Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4°C. Secondary antibody: anti-mouse IgG HRP antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4.

Quick Database Links

UniProt

P43694

RefSeq (Protein):NP_002043.2