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GATA4 Antibody
SKU: orb344532
Description
Images & Validation
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution range | ELISA: 1:2000-1:10,000, WB: 1:500-1:1000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 10D8.B10.D4 |
| Immunogen | GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein. |
| Purity | Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography. A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 1.0mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody
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Western Blot of Mouse anti-GATA4 Antibody. Lane 1: CCRF-CEM Lysate. Load: 25 µg per lane. Primary antibody: GATA-4 antibody at 1:500 overnight at 4°C. Secondary antibody: anti-mouse IgG HRP antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 44.5 kDa, 44.5 kDa for GATA4.
Quick Database Links
UniProt
RefSeq (Protein):NP_002043.2
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_002043.2 |
|---|
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
GATA4 Antibody (orb344532)
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