GATA4 Antibody

• Catalog Number: orb1524713
• Category: Antibodies
• Description: GATA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 392 and 442
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GATA4
• UniProt ID: P43694
• NCBI: NP_002043.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ASD2; GATA-binding factor 4; TACHD; TOF; transcrip
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human GATA4 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, SK-N-MC, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GATA4 antibody (orb1524713) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human GATA4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GATA4 antibody (orb1524713) used for IP at 6 µg per reaction. GATA4 was also immunoprecipitated by a previous lot of this antibody (orb1524713) and a second antibody against a different epitope of GATA4.

Detection of human GATA4 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-GATA4 (Cat. No. orb1524713) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB