GATA4 Antibody

• Catalog Number: orb1242655
• Category: Antibodies
• Description: GATA4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GATA4.
• Concentration: batch dependent
• Dilution range: GATA4 antibody can be used for detection of GATA4 by ELISA at 1:1562500. GATA4 antibody can be used for detection of GATA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 45 kDa
• Target: GATA4
• UniProt ID: Q5IFM8
• NCBI: NP_002043
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store GATA4 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: GATA4, ASD2, VSD1, TACHD
• Note: For research use only
• Application notes: GATA4 antibody can be used for detection of GATA4 by ELISA at 1:1562500. GATA4 antibody can be used for detection of GATA4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Mouse tumor at 1:1000 (Lanes: 1: 25 ug mouse tumor + shRNA1, 2: 25 ug mouse tumor + shRNA2, 3: 25 ug mouse tumor + shRNA3 4: 25 ug mouse tumor lysate).

Antibody used in WB on Human Jurkat 0.2-1 ug/ml.