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Catalog Number | orb18799 |
---|---|
Category | Antibodies |
Description | GAS 6/GAS6 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human GAS 6 recombinant protein (Position: M488-S660). Human GAS 6 shares 79.7% and 81.4% amino acid (aa) sequence identity with mouse and rat GAS 6, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 79677 MW |
UniProt ID | Q14393 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Growth arrest-specific protein 6;GAS-6;AXL recepto Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of THP-1 cells using anti-GAS 6 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of GAS 6 using anti-GAS 6 antibody.Lane 1:rat brain tissue;2:mouse brain tissue.
IF analysis of GAS 6 using anti-GAS 6 antibody. GAS 6 was detected in immunocytochemical section of A431 cells.
IHC analysis of GAS 6 using anti-GAS 6 antibody. GAS 6 was detected in paraffin-embedded section of mouse spleen tissues.
IHC analysis of GAS 6 using anti-GAS 6 antibody.GAS 6 was detected in paraffin-embedded section of rat spleen tissues.
ELISA, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rat |
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