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Catalog Number | orb2292922 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody raised against a partial recombinant GAPDH. |
Clonality | Monoclonal |
Species/Host | Mouse |
Isotype | IgG1 Kappa |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | GAPDH (NP_002037, 226 a.a. ~ 335 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Protein Sequence | GKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE |
Tested applications | ELISA, IP, WB |
Clone Number | 1G5 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | NP_002037 |
Detection limit for recombinant GST tagged GAPDH is approximately 1 ng/ml as a capture antibody.
GAPDH monoclonal antibody (M03), clone 1G5. Western Blot analysis of GAPDH expression in HeLa.
Immunoprecipitation of GAPDH transfected lysate using anti-GAPDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GAPDH MaxPab rabbit polyclonal antibody.
Western Blot detection against Immunogen (37.84 KDa).