GAPDH Antibody

• Catalog Number: orb68961
• Category: Antibodies
• Description: Mouse monoclonal antibody to GAPDH
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 1A10A10
• Tested applications: ELISA, ICC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human GAPDH expressed in E. Coli.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 37kDa
• Entrez: 2597
• Storage: 4°C; -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Ascitic fluid containing 0.03% sodium azide.
• Alternative names: G3PD; GAPD; MGC88685
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.

Western blot analysis of Hela (1), A549 (2), A431(3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GAPDH antibody.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.