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GAPDH Antibody
SKU: orb68961
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/5000- 1/20000; IHC: 1/200 - 1/1000; ICC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Monkey, Mouse, Rabbit, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 1A10A10 |
| Immunogen | Purified recombinant fragment of human GAPDH expressed in E. Coli. |
| Target | GAPDH |
| Molecular Weight | 37kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−G3PD; GAPD; MGC88685;Glyceraldehyde-3-phosphate dehydrogenase
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Confocal immunofluorescence analysis of methanol-fixed HepG2 (left) and Hela (right) cells using GAPDH antibody (green), showing cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
Western blot analysis of Hela (1), A549 (2), A431(3), MCF-7 (4), K562 (5), Jurkat (6), HL60 (7), SKN-SH (8) and SKBR-3 (9) cell lysate using GAPDH antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left) and kidney carcinoma (right), showing cytoplasmic localization using GAPDH antibody with DAB staining.
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
GAPDH Antibody (orb68961)
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