GAPDH Antibody

SKU: orb344436

Description

GAPDH antibody

Images & Validation

• Tested Applications: ELISA, IF, Multiplex Assay, WB
• Dilution range: IF: 1:100, WB: 1:1,000
• Reactivity: Human, Mouse, Rabbit
• Application Notes:
  Anti-GAPDH (Mouse) has been tested in ELISA and western blot. This product is suitable in IHC and IF. Specific conditions should be optimized by the end user. Expect a band of ~38kDa in size corresponding to Glyceraldehyde 3-Phosphate Dehydrogenase protein by Western blot in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgM
• Clone No.: 33F2.C8.G6.E3
• Immunogen: Anti-GAPDH Monoclonal Antibody was produced by repeated immunizations in mice with rabbit GAPDH protein.
• Purity: Anti-GAPDH Monoclonal Antibody is directed against rabbit Glyceraldehyde-3-Phosphate Dehydrogenase. The antibody is a total IgM fraction prepared from ascites fluid by selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Anti-GAPDH antibodies are expected to react with the following species based on 100% sequence homology: rabbit, human, and mouse. Reactivity against other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-GAPDH antibody, Loading Control Antibody, G3PDH, GAPD, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

Immunofluorescence Microscopy of Mouse Anti-Glyceraldehyde 3-Phosphate Dehydrogenase Antibody. Tissue: Human neuroblastoma SH-SY5Y cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: GAPDH antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Fluorescein mouse secondary antibody at 1:10000 for 45 min at RT. Localization: GAPDH is cytoplasmic. Staining: Anti-GAPDH (green), chicken antibody to neurofilament NF-H (red) and DNA (blue).

Western Blot of Mouse Anti-GAPDH Antibody. Lane 1: Molecular Weight. Lane 2: Glyceraldehyde-3-Phosphate-Dehydrogenase. Primary Antibody: Mouse Anti-GAPDH Antibody at 1 µg/ml. Secondary Antibody: Anti-Mouse IgG HRP. Block: BlockOut Buffer (p/n orb348644). Predicted MW: ~38kDa.

Western Blot of Mouse anti-Glyceraldehyde-3-Phosphate Dehydrogenase antibody. Lane 1: rat hippocampal lysate. Lane 2: none. Load: 10 µg per lane. Primary antibody: GAPDH antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~38kDa/~38kDa for GAPDH protein. Other band(s): splice variants and isoforms.

Quick Database Links

UniProt

P46406

RefSeq (Protein):NP_001075722.1