GAPDH Antibody (Biotin)

• Catalog Number: orb1238397
• Category: Antibodies
• Description: GAPDH Antibody (Biotin)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 12D3D5
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG
• Immunogen: Biotin-GAPDH antibody was raised against a 16 amino acid peptide near the carboxy terminus of human GAPDH.
• Concentration: 1 mg/mL
• Dilution range: Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Biotin
• MW: Predicted: 37 kDa Observed: 36 kDa
• Target: GAPDH
• UniProt ID: P04406
• NCBI: NP_002037
• Storage: Biotin-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: Biotin-GAPDH antibody is supplied in PBS containing 1% rAlbumin and 0.02% sodium azide.
• Alternative names: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, G
• Note: For research use only
• Application notes: Biotin-GAPDH antibody can be used for detection of GAPDH by Western blot at 1 - 2 μg/ml. Antibody validated: Western Blot in human, mouse, and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GAPDH in multiple cell and tissue lysates with Biotin-GAPDH antibody at 1 μg/ml. Lanes 1-20: 293, A431, A549, Daudi, HeLa, HepG2, Jurkat, K562, MOLT, 3T3, Raji, Ramos, U937, human brain, mouse brain, rat brain, rabbit brain, mouse lung, mouse liver, and rat liver, respectively.