gamma Tubulin antibody

• Catalog Number: orb44548
• Category: Antibodies
• Description: Mouse Monoclonal to gamma Tubulin.
• Clonality: Monoclonal
• Clone Number: TU-30
• Tested applications: FC, ICC, WB
• Reactivity: Bovine, Gallus, Human, Mouse, Plant, Porcine, Protozoa, Rat
• Isotype: Mouse IgG1
• Immunogen: C-terminal peptide of gamma-tubulin counjugated to KLH.
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Recommended dilution: 1-2 μg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: gamma-Tubulin
• Entrez: 7283
• UniProt ID: P23258
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-gamma-tubulin antibody
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution: 1-2 μg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells.Western blotting: Recommended dilution 1-2 μg/ml, reducing conditions.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells stained using anti-gamma-Tubulin (TU-30) purified antibody (concentration in sample 9 µg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of P19X1 mouse embryonal carcinoma cell line using anti-gamma-tubulin (TU-30) (detection by secondary antibody Goat anti-mouse Cy3). Nuclei were stained with DAPI (blue).

Immunocytochemistry staining of murine fibroblasts using anti-gamma-tubulin (TU-30; direct conjugate with Dyomics 547, red). Nuclei were stained with DAPI (blue).

Immunocytochemistry staining of microtubular networks in 3T3 mouse fibroblasts. A - metaphase; B - anaphase; C - telophase. Gamma-tubulin (red) stained with anti-gamma-tubulin (TU-30), alpha-tubulin (green) with polyclonal anti-alpha-tubulin antibody and nuclei with DAPI (blue).

Western blotting analysis of human gamma-tubulin using mouse monoclonal antibody TU-30 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-gamma-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for gamma-tubulin at approximately 46 kDa.

Western blotting analysis of differential reactivity of monoclonal antibodies to γ-tubulin with human γ-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human γ-tubulin 1 (γ-Tb1) or γ-tubulin 2 (γ-Tb2), probed with Abs to γ-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of γ-Tb1 or γ-Tb2 probed with Abs to γ-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.