gamma Sarcoglycan Rabbit Monoclonal Antibody

• Catalog Number: orb548395
• Category: Antibodies
• Description: gamma Sarcoglycan Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: AbS34
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human gamma Sarcoglycan
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Dilution range: WB 1:500-1:2000IHC 1:50-1:200IP 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 32379 MW
• UniProt ID: Q13326
• Storage: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
• Alternative names: Gamma-sarcoglycan;Gamma-SG;35 kDa dystrophin-assoc
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of gamma Sarcoglycan expression in Human skeletal muscle lysate.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-gamma Sarcoglycan antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.