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    GALNS antibody

    Catalog Number: orb340920

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb340920
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to GALNS
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenRecombinant full length protein of human Chondroitinase
    Dilution rangeWB: 1:500-1:2000
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetGALNS
    Entrez2588, 292073, 50917
    UniProt IDQ571E4, Q32KJ6, P34059
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesN-acetylgalactosamine-6-sulfatase; Chondroitinsulf
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    GALNS antibody

    Western blot analysis of Chondroitinase expression in MCF7 (A), HT29 (B), mouse kidney (C), mouse brain (D) whole cell lysates. (Predicted band size: 58 kD; Observed band size: 55 kD)

    GALNS antibody

    Immunofluorescent analysis of Chondroitinase staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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