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Catalog Number | orb669747 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to Galectin 9 |
Species/Host | Rat |
Clonality | Monoclonal |
Clone Number | RG9-35 |
Tested applications | Blocking, FA, FC, IP |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | This antibody was raised by immunising rats with recombinant mouse galectin-9. |
Concentration | 1 mg/ml |
Purity | Purified |
Conjugation | Unconjugated |
Target | Galectin 9 |
UniProt ID | O08573 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | ecalectin; Gal-9; galectin 9; Galectin-9; HUAT; le Read more... |
Note | For research use only |
Application notes | When this antibody was first generated and characterised, it was shown to bind with galectin-9 (Gal-9) but not with either galectin-1 or galectin-3. Furthermore, it was shown to be capable of blocking Tim-3-Fc binding to Gal-9, and inhibiting Gal-9-induced Th1 cell death in vitro (Fukushima et al., 2008; PMID: 18087160). This antibody has been used in FACS analyses. For instance, to describe a novel mechanism where Tim-3 binds HMGB-1 on virus-specific CD8+ Treg cells, suppressing proliferation of CD8+ T effector cells during acute adenoviral infection (Dolina et al., 2014; PMID: 24677194); and to indicate a crucial role for Tim-3 in phagocytosis of apoptotic cells and cross-presentation (Nakayama et al., 2009; PMID: 19224762). In addition, this antibody has been used in vivo to study the roles of Gal-9 in the development of experimental allergic conjunctivitis in mice (Fukushima et al., 2008; PMID: 18087160) and to demonstrate that constitutive expression of Gal-9 plays an immunosuppressive role in corneal allografts (Shimmura-Tomita et al., 2013; PMID: 23667648). This antibody was used in a cold immunoprecipitation (IP) assay to explore the binding of Gal-Nab1 and Gal-Nab2 to native murine gal-9, using a protein extract from murine thymus as the source of native Gal-9; RG9-35, was one of the antibodies used to isolate immune complexes formed between primary antibodies and proteins extracted from a mouse thymus for further analysis (Lhuillier et al., 2018; PMID: 30204750). |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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