Galectin 1 Antibody / LGALS1

SKU: orb606808

Description

Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Titering of the recombinant Galectin 1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: GAL1/2499R
• Immunogen: A human recombinant partial protein corresponding to amino acids 12-108 was used as the immunogen for the recombinant Galectin 1 antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human HeLa cell lysate with recombinant Galectin 1 antibody (clone GAL1/2499R). Expected molecular weight ~14 kDa.

Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with recombinant Galectin 1 antibody (clone GAL1/2499R). Expected molecular weight ~14 kDa.

IHC testing of FFPE human prostate carcinoma with recombinant Galectin 1 antibody (clone GAL1/2499R). Required HIER: boil tissue sections in 10 mM citrate buffer, pH6, for 10-20 min and allowed to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant Galectin 1 antibody (clone GAL1/2499R). These results demonstrate the foremost specificity of the GAL1/2499R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Galectin 1 antibody (clone GAL1/2499R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P09382