Galectin 1 Antibody / LGALS1

• Catalog Number: orb606808
• Category: Antibodies
• Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: GAL1/2499R
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A human recombinant partial protein corresponding to amino acids 12-108 was used as the immunogen for the recombinant Galectin 1 antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Galectin 1 antibody is available for research use only.
• UniProt ID: P09382
• Storage: Store the recombinant Galectin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the recombinant Galectin 1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human HeLa cell lysate with recombinant Galectin 1 antibody (clone GAL1/2499R). Expected molecular weight ~14 kDa.

Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with recombinant Galectin 1 antibody (clone GAL1/2499R). Expected molecular weight ~14 kDa.

IHC testing of FFPE human prostate carcinoma with recombinant Galectin 1 antibody (clone GAL1/2499R). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min and allowed to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using recombinant Galectin 1 antibody (clone GAL1/2499R). These results demonstrate the foremost specificity of the GAL1/2499R mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free recombinant Galectin 1 antibody (clone GAL1/2499R) as confirmation of integrity and purity.