Galectin 1 Antibody / LGALS1

• Catalog Number: orb385945
• Category: Antibodies
• Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GAL1/1831
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, lambda
• Immunogen: A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Galectin 1 antibody is available for research use only.
• UniProt ID: P09382
• Storage: Store the Galectin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Galectin 1 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) JEG-3 and 2) K562 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.

Western blot testing of human HeLa cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.

Western blot testing of human 1) HeLa, 2) K562 and 3) HEK293 cell lysate with Galectin 1 antibody (clone GAL1/1831). Expected molecular weight ~14 kDa.

Immunofluorescent staining of permeabilized human HeLa cells with Galectin 1 antibody (clone GAL1/1831, green) and Reddot nuclear stain (red).

IHC testing of FFPE human prostate carcinoma with Galectin 1 antibody (clone GAL1/1831). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using Galectin 1 antibody (clone GAL1/1831). These results demonstrate the foremost specificity of the GAL1/1831 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free Galectin 1 antibody (clone GAL1/1831) as confirmation of integrity and purity.