Galectin 1 Antibody / LGALS1

• Catalog Number: orb385945
• Category: Antibodies
• Description: Galectin 1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kDa participating in a variety of normal and pathological processes, including cancer progression. Galectin 1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin 1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin 1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GAL1/1831
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, lambda
• Immunogen: A human partial recombinant protein corresponding to amino acids 12-108 was used as the immunogen for the Galectin 1 antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Galectin 1 antibody is available for research use only.
• UniProt ID: P09382
• Storage: Store the Galectin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the Galectin 1 antibody may be required for optimal performance.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human prostate carcinoma using Galectin 1 antibody

Western blot analysis of human HeLa(lane 1), K562(lane 2) and HEK293(lane 3) cell lysate using Galectin 1 antibody